Biosynthesis of Bacterial Lipopolysaccharide

Biosynthesis of bacterial lipopolysaccharide. VII. Enzymatic formation of the first intermediate in biosynthesis of the O-antigen of Salmonella typhimurium.

The initial step in biosynthesis of the O-antigen of Salmonella typhimurium is the formation of a lipid-linked derivative of galactose l-phosphate, according to the reaction UDP-galactose + P-lipid ti galactose-1 -PP-lipid + UMP. The enzyme activity has been detected in the cell envelope fraction of Citrobocter strain 139 (04, 5, 12) as well as in wild type S. typhimurium strain LT-2, but is mo...

Biosynthesis of bacterial lipopolysaccharide. I. Enzymatic incorporation of galactose in a mutant strain of Salmonella.

Although the mechanism of biosynthesis of homopolysaccharides, such as glycogen,' dextran,2 and starch,3 has been studied in some detail, relatively little attention has been given to the biosynthesis of the heteropolymeric mucopolysaccharides and lipopolysaccharides elaborated by animals and microorganisms. A promising approach to this problem has arisen out of recent work in several laborator...

The AS87_04050 Gene Is Involved in Bacterial Lipopolysaccharide Biosynthesis and Pathogenicity of Riemerella anatipestifer

Riemerella anatipestifer is reported worldwide as a cause of septicemic and exudative diseases of domestic ducks. In this study, we identified a mutant strain RA2640 by Tn4351 transposon mutagenesis, in which the AS87_04050 gene was inactivated by insertion of the transposon. Southern blot analysis indicated that only one insertion was found in the genome of the mutant strain RA2640. SDS-PAGE f...

Bacterial DNA and Lipopolysaccharide

Initiation of Methylglucose Lipopolysaccharide Biosynthesis in Mycobacteria

BACKGROUND Mycobacteria produce two unique families of cytoplasmic polymethylated polysaccharides -- the methylglucose lipopolysaccharides (MGLPs) and the methylmannose polysaccharides (MMPs) -- the physiological functions of which are still poorly defined. Towards defining the roles of these polysaccharides in mycobacterial physiology, we generated knock-out mutations of genes in their putativ...